A technique of analytical chemistry used to separate and purify a biological molecule from a mixture, based on the attraction of the molecule of interest to a particular ligand which has been previously attached to a solid, inert substance. The mixture is passed through a column containing the ligand attached to the stationary substance, so that the molecule of interest stays within the column while the rest of the mixture continues through to the end. Then, a different chemical is flushed through the column to detach the molecule from the ligand and bring it out separately from the rest of the mixture.
Selected affinity chromatography links:
© 1997-2006 Healthboard.com.
Healthboard.com is a purely
informational website, and should not be used as a substitute
for professional legal, medical or technical advice.