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Gram stain

An important method for staining bacteria developed by Christian Gram in 1884. The bacteria are placed as a smear on a slide, then air-dried, then stained first with crystal violet dye and then with Gram's iodine, then washed with 95% ethanol, flooded with safranin or fuchsin (red dyes) and air-dried again. If the bacteria retain the purple-blue stain on their cell walls, they're classified as Gram-positive; if they don't retain the crystal violet but take the red counterstain, they're Gram-negative. This classification is important because the reaction to Gram stain correlates in many cases with the bacteria's vulnerability to certain antibiotics. The process only takes a few minutes, making it ideal for medical clinics. If a clinician considers data from the Gram stain along with his/her medical examination of a sick patient, the clinician can get a pretty good idea of the pathogen that is infecting the patient and then start a regimen of antibiotics. In a clinical setting, the Gram stain is followed up by a bacterial culture (which takes three to six days to complete).

Selected Gram stain links:

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