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homopolymer tailing

A lab technique where a nucleic acid homopolymer (a long chain of the same nucleotide over and over) is attached to the end of a piece of DNA as a part of DNA cloning. It is especially useful in cDNA cloning to get the cDNA inserted into the cloning vector -- a nucleic acid homopolymer which contains the complementary nucleotide to the cDNA tail is attached to the vector, which then pairs with the tail on the cDNA.

Selected homopolymer tailing links:

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