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Southern blot

A technique used for searching for a specific DNA fragment. The process is as follows: Separate DNA fragments by gel electrophoresis change pH of gel to basic, thus allowing disruption of H-bonds blot gel with nitrocellulose paper heat paper so as to fix DNA fragments probe with labeled messenger RNA or cDNA wash complementary mRNA/cDNA fragments will have hybridized.

Selected Southern blot links:

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